abstract：AIMS:To compare a range of enrichment broths and enrichment temperatures for the isolation of Escherichia coli O157 by immunomagnetic separation (IMS) from sandy, loam and clay soils. METHODS AND RESULTS:Soils were spiked with cocktails of four atoxigenic strains of E. coli O157 and four strains of commensal E. coli. ...

journal_title：Letters in applied microbiology

authors： Hepburn NF,MacRae M,Johnston M,Mooney J,Ogden ID

更新日期：2002-01-01 00:00:00

abstract：AIMS:The effect of high organic loading rate (OLR) on the physical characteristics of aerobic granules was studied. METHODS AND RESULTS:Two column-type sequential aerobic sludge blanket reactors were fed with either glucose or acetate as the main carbon source, and the OLR was gradually raised from 6 to 9, 12 and 15 k...

journal_title：Letters in applied microbiology

authors： Moy BY,Tay JH,Toh SK,Liu Y,Tay ST

更新日期：2002-01-01 00:00:00

abstract：AIMS:Observation of the dependence of alpha-L-rhamnosidase activity on pH and temperature and the capability to hydrolyse concentrated naringin solutions and hesperidin suspensions of enzyme complexes produced by several fungi. METHODS AND RESULTS:The enzymes were produced by several wild strains of mesophilic fungi g...

journal_title：Letters in applied microbiology

authors： Scaroni E,Cuevas C,Carrillo L,Ellenrieder G

更新日期：2002-01-01 00:00:00

abstract：AIMS:To isolate a bacterium capable of degrading 3 beta-acetoxypregna-5,16-diene-20-one (16-DPA) to androsta-1,4-diene-3,17-dione (ADD) and to decipher the biodegradation pathway. METHODS AND RESULTS:Isolation on mineral salt agar containing 16-DPA as sole carbon source yielded two bacteria identified as Pseudomonas d...

journal_title：Letters in applied microbiology

authors： Patil S,Shukla A,Shinde K,Banerjee T

更新日期：2002-01-01 00:00:00

abstract：AIMS:The objective of this study was to subtype Arcobacter butzleri isolates using RAPD-PCR. METHODS AND RESULTS:Thirty-five A. butzleri isolates obtained from chicken carcasses were examined. PCR-mediated DNA fingerprinting technique with primers of the variable sequence motifs was used to detect polymorphism within ...

journal_title：Letters in applied microbiology

authors： Atabay HI,Bang DD,Aydin F,Erdogan HM,Madsen M

更新日期：2002-01-01 00:00:00

abstract：AIMS:To evaluate the genetic diversity within the species Paenibacillus polymyxa. METHODS:Southern hybridization was performed on 102 strains of P. polymyxa using DNA from the phage IPy1 as a probe. RESULTS:All 102 strains hybridized to phage IPy1 DNA. Data from different hybridization patterns obtained were used to ...

journal_title：Letters in applied microbiology

authors： Santos SC,Rodrigues Coelho MR,Seldin L

更新日期：2002-01-01 00:00:00

abstract：AIMS:The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. METHODS AND RESULTS:Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cult...

journal_title：Letters in applied microbiology

authors： Eyigor A,Carli KT,Unal CB

更新日期：2002-01-01 00:00:00

abstract：AIMS:We propose to apply the Wirtz-Conklin staining technique to evaluate spore germination. METHODS AND RESULTS:Spores at different stages of germination were stained with modified spore stain (Wirtz-Conklin) and evaluated for staining properties. Bacillus spores suspended in deionized water, which does not support g...

journal_title：Letters in applied microbiology

authors： Hamouda T,Shih AY,Baker JR Jr

更新日期：2002-01-01 00:00:00

abstract：AIMS:This work was carried out to study the acid production by Lactococcus lactis subsp. lactis strains isolated from goat's milk and goat cheese (Valdeteja variety) in order to select a suitable starter culture for industrial goat cheese manufacturing. METHODS AND RESULTS:The titrable acidity of 45 Lactococcus lactis...

journal_title：Letters in applied microbiology

authors： Alonso-Calleja C,Carballo J,Capita R,Bernardo A,García-López ML

更新日期：2002-01-01 00:00:00

abstract：AIMS:From a survey of submerged culture of edible mushrooms, a high pigment-producing fungus Paecilomyces sinclairii was selected and its optimal culture conditions investigated. METHODS AND RESULTS:The optimal culture conditions for pigment production were as follows: inoculum age, 3 d; temperature, 25 degrees C; ini...

journal_title：Letters in applied microbiology

authors： Cho YJ,Park JP,Hwang HJ,Kim SW,Choi JW,Yun JW

更新日期：2002-01-01 00:00:00

abstract：AIMS:The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes. METHODS AND RESULTS:30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AG...

journal_title：Letters in applied microbiology

authors： Zhang GW,Kotiw M,Daggard G

更新日期：2002-01-01 00:00:00

abstract：AIMS:To investigate a lactococcal plasmid responsible for a reduction in growth rate of its host cell. METHODS AND RESULTS:Lactococcus lactis subsp. lactis biovar. diacetylactis DRC1 carries a high number of plasmids. The DRC1 wild-type strain was found to grow more slowly than a plasmid-free derivative of DRC1. The p...

journal_title：Letters in applied microbiology

authors： Kobayashi M,Nomura M,Fujita Y,Okamoto T,Ohmomo S

更新日期：2002-01-01 00:00:00

abstract：AIMS:The antigen 85 complex (Ag85) from Mycobacterium tuberculosis consists of three abundantly secreted proteins (FbpA, FbpB and FbpC2) which play a key role in the pathogenesis of tuberculosis and also exhibit cell wall mycolyltransferase activity. A related protein with similarity to the Ag85 complex was recently an...

journal_title：Letters in applied microbiology

authors： Kremer L,Maughan WN,Wilson RA,Dover LG,Besra GS

更新日期：2002-01-01 00:00:00

abstract：UNLABELLED:AIMIS: Draft WHO guidance is likely to suggest a calculated 95th percentile method, rather than percentage exceedence, in assessing compliance of bathing waters with microbiological standards. This study set out to determine whether this was an appropriate development. METHODS AND RESULTS:A series of Monte ...

journal_title：Letters in applied microbiology

authors： Hunter PR

更新日期：2002-01-01 00:00:00

abstract：AIMS:To investigate the influence of the growth phase, growth temperature, storage time, pH and aw of the treatment medium on the resistance of Listeria monocytogenes to pulsed electric fields (PEF). METHODS AND RESULTS:Square wave pulses of 2 micros at a frequency of 1 Hz and 25 and 28 kV cm(-1) were used. Cells were...

journal_title：Letters in applied microbiology

authors： Alvarez I,Pagán R,Raso J,Condón S

更新日期：2002-01-01 00:00:00

abstract：AIMS:To investigate interactions, if any, between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in modified Pine's medium (Pine et al. 1989). METHODS AND RESULTS:Modified Pine's medium containing 0, 0.044, 0.088 or 0.176 g l(-1) ferric ammonium citrate, and 0 or 1 mM ...

journal_title：Letters in applied microbiology

authors： Dykes GA,Dworaczek M

更新日期：2002-01-01 00:00:00

abstract：AIMS:To identify and characterize amino acid transport in Leuconostoc mesenteroides. METHODS AND RESULTS:The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273. Systems were operative under physiological conditions of growth, energy dependent and differed from peptide transp...

journal_title：Letters in applied microbiology

authors： Gendrot F,Foucaud-Scheunemann C,Ferchichi M,Hemme D

更新日期：2002-01-01 00:00:00

abstract：AIMS:The present work aimed to design an optimized medium to yield a higher production of glycosides by Pseudoalteromonas issachenkonii KMM 3549(T). METHODS AND RESULTS:Higher levels of fucoidan hydrolase, alginase, laminaranase and b-N-acetylglucosaminidase production were obtained with peptone concentrations ranging...

journal_title：Letters in applied microbiology

authors： Alexeeva YV,Ivanova EP,Bakunina IY,Zvaygintseva TN,Mikhailov VV

更新日期：2002-01-01 00:00:00

abstract：AIMS:To investigate the incidence of Campylobacter and Salmonella contamination associated with supermarket and butchers' shop chicken and related packaging. METHOD AND RESULTS:Three hundred raw samples (whole chicken, chicken breast with skin or chicken pieces) were purchased on a monthly basis for seven months. Pack...

journal_title：Letters in applied microbiology

authors： Harrison WA,Griffith CJ,Tennant D,Peters AC

更新日期：2001-12-01 00:00:00

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00

abstract：AIMS:Species-specific PCR was applied to identify Lactobacillus brevis and the sensitivity and the specificity of the protocol were determined. METHODS AND RESULTS:Strains of Lact. brevis obtained from foods, particularly dairy products, and various strain collections, were identified by PCR using primers which amplif...

journal_title：Letters in applied microbiology

authors： Guarneri T,Rossetti L,Giraffa G

更新日期：2001-11-01 00:00:00

abstract：AIMS:To investigate the genetics of dimethoate degradation in Pseudomonas aeruginosa MCMB-427. METHODS AND RESULTS:Pseudomonas aeruginosa MCMB-427 demonstrated the ability to degrade dimethoate, a synthetic organophosphate insecticide. Total DNA preparation of MCMB-427 revealed the presence of a 6.6 kbp plasmid (desig...

journal_title：Letters in applied microbiology

authors： Deshpande NM,Dhakephalkar PK,Kanekar PP

更新日期：2001-10-01 00:00:00

abstract：AIMS:The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin). METHODS AND RESULTS:Of the polymers tested, PE and PE + PEO did not exhibit an...

journal_title：Letters in applied microbiology

authors： Cutter CN,Willett JL,Siragusa GR

更新日期：2001-10-01 00:00:00

abstract：AIMS:The optimal culture conditions for endoinulinase production using chicory roots were studied in shake-flask culture. METHODS AND RESULTS:Much higher enzyme production was achieved with Xanthomonas sp. (15 U ml(-1)) than with Pseudomonas sp. (3 U ml(-1)). Optimized culture conditions of Xanthomonas sp. for endoinu...

journal_title：Letters in applied microbiology

authors： Park JP,Yun JW

更新日期：2001-09-01 00:00:00

abstract：AIMS:A rapid and simple method for enumerating uninjured and sublethally injured bacterial cells, the twofold dilution method (2FD), was developed and evaluated. METHODS AND RESULTS:Following twofold serial dilution of samples in a 96 well microtiter plate, double strength selective broth or nonselective broth was add...

journal_title：Letters in applied microbiology

authors： Kang DH,Siragusa GR

更新日期：2001-09-01 00:00:00

abstract：AIMS:Oenococcus oeni is a lactic acid bacterium used in wine fermentation. Two open reading frames (orfB and orfC) were identified in the upstream region of the hsp18 gene, encoding the small heat-shock protein Lo18. Expression of these genes in conditions of acid stress was studied in Escherichia coli. METHODS AND RE...

journal_title：Letters in applied microbiology

authors： Morel F,Delmas F,Jobin MP,Diviès C,Guzzo J

更新日期：2001-08-01 00:00:00

abstract：AIMS:The study was carried out to evaluate the use of randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) as a method for the identification of lactobacilli isolated from meat products. METHODS AND RESULTS:RAPD-PCR with primers M13 and D8635 was applied to the identification and intraspecific diffe...

journal_title：Letters in applied microbiology

authors： Andrighetto C,Zampese L,Lombardi A

更新日期：2001-07-01 00:00:00

abstract：AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask cu...

journal_title：Letters in applied microbiology

authors： Park JP,Kim SW,Hwang HJ,Yun JW

更新日期：2001-07-01 00:00:00

abstract：AIMS:To establish species-specific PCR and DNA probe methods for Streptococcus salivarius and to clarify the distribution of dextranase in oral isolates of Strep. salivarius. METHODS AND RESULTS:A pair of PCR primers and a DNA probe were designed based on the nucleotide sequence of the dextranase gene of Strep. saliva...

journal_title：Letters in applied microbiology

authors： Igarashi T,Yano Y,Yamamoto A,Sasa R,Goto N

更新日期：2001-06-01 00:00:00

abstract：AIMS:Enzyme kinetics of purified laccases from six different Pleurotus ostreatus strains were determined in the oxidation of syringaldazine, guaiacol and ABTS. METHODS AND RESULTS:Significant differences in the kinetic constants were found. Catalytic activity (kcat) ranged from 19 to 941 U mg(-1) for syringaldazine, f...

journal_title：Letters in applied microbiology

authors： Tinoco R,Pickard MA,Vazquez-Duhalt R

更新日期：2001-05-01 00:00:00

abstract：AIMS:To compare the fermentative capacity of wild and domesticated isolates of the genus Saccharomyces. METHODS AND RESULTS:The fermentative capacity of yeasts from a variety of wild and domesticated sources was tested in synthetic dough media that mimic major bread dough types. Domesticated yeast strains were found t...

journal_title：Letters in applied microbiology

authors： Bell PJ,Higgins VJ,Attfield PV

更新日期：2001-04-01 00:00:00

abstract：AIMS:An agar medium containing a range of related chlorophenoxyalkanoic acid herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D), 2-methyl-4-chlorophenoxyacetic acid (MCPA), racemic mecoprop, (R)-mecoprop and racemic 2,4-DP (2-(2,4-dichlorophenoxy) propionic acid) was developed to assess the catabolic activity of a rang...

journal_title：Letters in applied microbiology

authors： Smejkal CW,Vallaeys T,Burton SK,Lappin-Scott HM

更新日期：2001-04-01 00:00:00

abstract：:To investigate the potential usefulness of u.v. germicidal irradiation (UVGI) in preventing the spread of Burkholderia cepacia, an important pathogen in cystic fibrosis (CF), the in-vitro susceptibility of B. cepacia to UVGI was determined. Five strains were exposed to UVGI from a 7.2-W source. Burkholderia cepacia wa...

journal_title：Letters in applied microbiology

authors： Cairns G,Kerr KG,Beggs CB,Sleigh PA,Mooney L,Keig P,Donnelly JK

更新日期：2001-03-01 00:00:00

abstract：:Leuconostoc strains were characterized according to their antibiotic susceptibilities, carbohydrate fermentation profiles, sucrase activity patterns and plasmid content. All the strains tested were resistant to the antibiotics sulphathiazole, trimethoprim and vancomycin, and could be separated into two groups based on...

journal_title：Letters in applied microbiology

authors： Holt SM,Al-Sheikh H,Shin KJ

更新日期：2001-03-01 00:00:00

abstract：:The L-2,3-butanediol dehydrogenase (L-BDH) gene of Brevibacterium saccharolyticum was strongly expressed in Escherichia coli using the tac promoter. However, the stereospecificity of the resulting L-BDH was reduced. The region upstream of the meso-BDH gene of Klebsiella pneumoniae was also involved in the expression o...

journal_title：Letters in applied microbiology

authors： Ui S,Takusagawa Y,Ohtsuki T,Mimura A,Ohkuma M,Kudo T

更新日期：2001-02-01 00:00:00

abstract：AIMS:Saccharomyces cerevisiae was used for studying the physiological effects of hydrostatic pressure. METHODS AND RESULTS:The effects of hydrostatic pressure on the ultrastructure of wild-type and trehalose-6-phosphate synthase (tps1) mutant cells were investigated by transmission electron microscopy. Pressure induce...

journal_title：Letters in applied microbiology

authors： Fernandes PM,Farina M,Kurtenbach E

更新日期：2001-01-01 00:00:00

abstract：:Enterococci were isolated from faecal droppings of chickens in broiler and layer farms and the susceptibilities to nine therapeutic antimicrobial agents and six growth-promoting antibiotics were determined by the agar dilution method. Resistance to therapeutic antimicrobial agents such as ampicillin, clindamycin, eryt...

journal_title：Letters in applied microbiology

authors： Yoshimura H,Ishimaru M,Endoh YS,Kojima A

更新日期：2000-12-01 00:00:00

abstract：:Salmonella enteritidis-inoculated poultry sausages were pressurized at 500 MPa by combining different times (10 and 30 min) and temperatures (50, 60 and 70 degrees C) or heat treated with the same temperature-time combinations and a standard cooking (75 degrees C for 30 min). Counts of Salm. enteritidis and mesophilic...

journal_title：Letters in applied microbiology

authors： Yuste J,Pla R,Mor-Mur M

更新日期：2000-11-01 00:00:00

abstract：:The cell surface hydrophobicity of micro-organisms is a characteristic that has been associated with the colonization of mammalian epithelia and with their capacity to induce diseases. Melatonin is a hormone produced by the pineal gland that affects the immune response mechanism. This study investigated, as an express...

journal_title：Letters in applied microbiology

authors： Uberos J,Molina A,Liébana J,Augustin MC,Muñoz A

更新日期：2000-10-01 00:00:00

abstract：:A study was made of the incidence of Campylobacter upsaliensis among dogs and cats suffering from acute or chronic diarrhoea; 225 dogs and cats were examined and 51 strains were identified, 16 (7%) of which were Camp. upsaliensis. When rectal swabs were taken from a control group of 126 dogs and cats without clinical ...

journal_title：Letters in applied microbiology

authors： Steinhauserova I,Fojtikova K,Klimes J

更新日期：2000-09-01 00:00:00